CRL-2095 CAL 27 人舌鱗癌細(xì)胞
| ATCC® Number: | CRL-2095™ | Price: | |
| Designations: | CAL 27 |
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| | Depositors: | C Cardona |
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| | Biosafety Level: | 1 |
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| | Shipped: | frozen |
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| | Medium & Serum: | See Propagation |
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| | Growth Properties: | adherent |
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| | Organism: | Homo sapiens （human） |
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| | Morphology: | epithelial
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| | Source: | Organ: tongue
Disease: squamous cell carcinoma |
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| | Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. |
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| | Isolation: | Isolation date: 1982 |
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| | Tumorigenic: | Yes |
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| | DNA Profile （STR）: | Amelogenin: X CSF1PO: 10,12 D13S317: 10,11 D16S539: 11,12 D5S818: 11,12 D7S820: 10 THO1: 6,9.3 TPOX: 8 vWA: 14,17 CRL-2095 CAL 27 人舌鱗癌細(xì)胞 |
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| | Cytogenetic Analysis: | aneuploid; modal number = 43 |
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| | Age: | 56 years |
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| | Gender: | male |
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| | Ethnicity: | Caucasian |
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| | Comments: | CAL 27 cells were resistant to treatment with VDS （vindesine sulfate）, CDP （cis-platinum） or ACTD （actinomycin D）. A culture submitted to the ATCC in December 1993 was found to be contaminated with mycoplasma. Progeny were cured by a 21-day treatment with BM Cycline. |
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| | Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C |
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| | Subculturin |
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| | Preservation: | Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor temperature |
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| | Doubling Time: | 35 hrs |
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| | Related Products: | Recommended medium （without the additional supplements or serum described under ATCC Medium）:ATCC 30-2002 recommended serum:ATCC 30-2020 |
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| | References: | 23521: Gioanni J, et al. Two new human tumor cell lines derived from squamous cell carcinomas of the tongue: establishment, characterization and response to cytotoxic treatment. Eur. J. Cancer Clin. Oncol. 24: 1445-1455, 1988. PubMed: 3181269
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